
Diagnostic Cytogenetics
- Chromosome Analysis / Karyotyping
- Chromosome Breakage Studies
- Mutagen induced chromosome sensitivity studies to evaluate the DNA repair proficiency
- Cytokinesis-block micronuclei assay to quantify the extend of somatic damages
Specimen Types for Chromosome Analysis / Karyotyping
- Peripheral Blood
- Bone Marrow Aspirate
- Leukemic Blood
- Products of Conception
- Intrauterine death/aborted foetus
- Each specimen must be clearly labeled with at least two patient identifiers such as patient name, age, sex, etc.
- Each specimen must be accompanied by a paper test requisition including the following: First and last name, birth date, gender, name of referring physician, originating lab or clinic, clinical indication and tests ordered.
- The clinical indication is required for appropriate cell culture parameters to be chosen
- Samples should be sent as soon as possible to the Cytogenetics Laboratory with same-day or overnight transport preferable.
- Samples should never be frozen or placed on ice
- Aseptically draw 1-3 ml venous blood into a sodium-heparin vacuutainer and mix well.
- Ensure accurate and clear labeling of the blood sample with patient information and collection details.
- Avoid using EDTA or other blood-thinning agents, and do not open the container.
- Store and transport the blood sample within recommended temperature and handling guidelines for reliable analysis.
- Transfer 1-2 ml of aspirate into a sodium-heparin vacutainer and mix well.
- Ensure aseptic conditions during the aspiration procedure.
- Accurately label the vacutainer with patient details and collection information
- Avoid using EDTA or other blood-thinning agents, and do not open the container.
- Ascetically transfer two or three tissue bits to a sterile bottle containing normal saline or in container provided by Genetika
- Kits with tissue transport media and a requisition form are available from the laboratory for convenient sampling and transport.
In cases of fetal demise, the placenta maintains cell viability longer due to maternal blood circulation; therefore, placental specimens are more likely to yield cell growth and therefore a cytogenetic result.
- Ascetically transfer two or three tissue bits placenta from near the umbilical cord insertion site containing chorionic villi to a sterile bottle containing normal saline or in container provided by Genetika.
- Kits with tissue transport media and a requisition form are available from the laboratory for convenient sampling and transport.